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Leja slides - 12 µm


  • 12 μm 2 lateral wells – 4 x 25

Leja slides are the most accurate fixed-depth slides on the market. Leja calibrated slides provide the accuracy and precision needed to reliably analyze semen. Leja slides facilitate analyses standardization.  

Overview

The Leja slide, more precise and reliable than its competitors, perfectly meets the requirements of CASA systems. It allows the standardization of analyses for repeatable and objective measurements.

This is why it is widely used by seed production centres, veterinarians and research centres.

High technology and precision

The accuracy of semen analysis is essential because it has a direct impact on the dilution rate calculated by CASA, which impacts the economic result.

The accuracy of a calibrated slide is related to the consistency of the chamber height. Leja has developed a production technique that allows it to certify a constant height. Leja is the only manufacturer able to issue this certification.

To achieve this, Leja has implemented a drastic quality control:

  • control and traceability of raw materials
  • toxicity control
  • height control
  • 18-point visual inspection....


High-tech materials

The high quality of the glass used gives a true picture of the quality of the seed read at CASA. The production of the blades in clean rooms limits any risk of dust being deposited on the blade, which avoids reading errors and makes the analysis even safer.

All the elements used are non-spermicidal and a toxicity control on the seed is carried out on each batch. A certificate of analysis is issued for each batch.

Control of chamber filling

Filling the chamber is one of the most important steps for reliable counting and sperm motility control. To ensure perfect reproducibility, the filling of the chambers by capillary action is optimized thanks to an exclusive coating. The sperm do not stick to the blade and the distribution of the seed is uniform, without the formation of bubbles.

These specificities favour the standardization of the procedure and the precision of the measurements for a better return on investment of the user.

  • The sample has to be liquefied and mixed homogeneously.
  • Avoid crystallization by leaving the sample stationary for a too long time.
  • Put a drop of the sample into the chamber and count the sperm heads as follows: 10 squares of the field are counted like blood cells in a haemocytometer. This number represents the concentration of spermatozoa in millions per millilitre.
  • When counting a sample of oligozoosperms all 100 squares have to be counted. Adding “00 000” to that number, you will get the concentration of the spermatozoa per millilitre.
  • Immotile spermatozoa are to be counted in a defined number of squares. Afterwards the motile spermatozoa are counted and classified into four categories from a) “fast progressive” to d) “no motility”. This procedure is to be repeated in different areas of the grid to have the quality and motility to be calculated on base of this data in percent.

Title

  • The Leja chamber is easy to use and you gain results fast and easy. The number of counted spermatozoa for each 10-field-grid is their concentration in millions/ml. For the calculation no conversion factors are needed.
  • Optimal depth. The meagre depth of only 10 µm ist especially adapted to the size of the spermatozoa wich allows them to diffuse evenly in a monolayer. They can move about freely in a single focal plane without the picture to bedome indistinct.
  • The grid is located in the cover glass. You don’t need a grid in your ocular.
  • A dilution of the sample is not needed as the analysis is made with the original sample in its natural seminal fluid or in a concentrate.
  • The analysis is made very fast meaning the patient does not have to wait too long for the results.
  • The analysis is precise because the steps you usually have to undergo with a haemocytometer are omitted and you are always working under reproducible conditions. Uneven pressure on the cover glass does not lead to mistakes.


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